Mechanism by Which Orally Administered Beta-1,3-Glucans Enhance the Tumoricidal Activity of AntiTumor Monoclonal Antibodies in Murine Tumor Models
The Journal of Immunology. 2004: 173: 797-806.
Feng Hong, Jun Yan, Jarek T. Baran, Daniel J. Allendorf, Richard D. Hansen, Gary R. Ostroff, Pei Xiang Xing, Nai-Kong V. Cheung, and Gordon D. Ross
Antitumor mAb bind to tumors and activate complement, coating tumors with iC3b. Intravenously administered yeast ß-1,3; 1,6-glucan functions as an adjuvant for antitumor mAb by priming the inactivated C3b (iC3b) receptors (CR3; CD11b/CD18) of circulating granulocytes, enabling CR3 to trigger cytotoxicity of iC3b-coated tumors. Recent data indicated that barley beta-1,3; 1,4-glucan given orally similarly potentiated the activity of antitumor mAb, leading to enhanced tumor regression and survival. This investigation showed that orally administered yeast ß-1,3;1,6-glucan functioned similarly to barley beta-1,3;1,4-glucan with antitumor mAb. With both oral beta-1,3-glucans, a requirement for iC3b on tumors and CR3 on granulocytes was confirmed by demonstrating therapeutic failures in mice deficient in C3 or CR3. Barley and yeast beta-1,3-glucan were labeled with fluorescein to track their oral uptake and processing in vivo. Orally administered ß-1,3-glucans were taken up by macrophages that transported them to spleen, lymph nodes, and bone marrow. Within the bone marrow, the macrophages degraded the large ß-1,3-glucans into smaller soluble ß-1,3-glucan fragments that were taken up by the CR3 of marginated granulocytes. These granulocytes with CR3-bound ß-1,3-glucan-fluorescein were shown to kill iC3b-opsonized tumor cells following their recruitment to a site of com- plement activation resembling a tumor coated with mAb.
Published at wellmune.com on 19 July 2004